Article
DNA microarrays based on noncovalent oligonucleotide attachment and hybridization in two dimensions.
Analytical biochemistry - 15 May 2001
Belosludtsev Y, Iverson B, Lemeshko S, Eggers R, Wiese R, Lee S, Powdrill T, Hogan M
Abstract excerpt
Short oligonucleotide probes have been linked to a solid support by simple electrostatic adsorption onto a positively charged surface film. Attachment was obtained by microfluidic application of unmodified oligonucleotides in distilled water onto amino-silanized glass. It has been demonstrated that an extremely stable monolayer of oligonucleotide is obtained by this method, at a density of about 10(11)...
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