Article
Attachment of oligonucleotide probes to poly carbodiimide-coated glass for microarray applications.
Nucleic acids research - 23 Apr 2004
Kimura Naoki, Oda Ryuichi, Inaki Yoshiaki, Suzuki Osamu
Abstract excerpt
Oligonucleotide-based DNA microarrays are becoming increasingly useful tools for the analysis of gene expression and single nucleotide polymorphisms (SNPs). Here, we present a method that permits the manufacture of microarrays from non-modified oligonucleotides on a poly carbodiimide-coated glass surface by UV-irradiation. The use of UV-irradiation facilitates an increase in the level of signal intensity, but it...
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