Article
Minisequencing on oligonucleotide microarrays: comparison of immobilisation chemistries.
Nucleic acids research - 1 Jul 2001
Lindroos K, Liljedahl U, Raitio M, Syvänen A C
Abstract excerpt
In the microarray format of the minisequencing method multiple oligonucleotide primers immobilised on a glass surface are extended with fluorescent ddNTPs using a DNA polymerase. The method is a promising tool for large-scale single nucleotide polymorphism (SNP) detection. We have compared eight chemical methods for covalent immobilisation of the oligonucleotide primers on glass surfaces. We included both...
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