Article
Direct removal in the mouse of a floxed neo gene from a three-loxP conditional knockout allele by two novel approaches.
Genesis (New York, N.Y. : 2000) - 1 May 2001
Xu X, Li C, Garrett-Beal L, Larson D, Wynshaw-Boris A, Deng C X
Abstract excerpt
The presence in an intron of the ploxP-neo-loxP cassette often results in severe interference with gene expression. Consequently, many investigators selectively remove the ploxP-neo-loxP cassette by transient expression of Cre in ES cells. Although effective, the added manipulation of the ES cells may reduce the likelihood that a clone will be able to transmit via the germline. Therefore, we developed two novel...
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