Article
Cre-mediated germline mosaicism: a new transgenic mouse for the selective removal of residual markers from tri-lox conditional alleles.
Nucleic acids research - 1 Mar 2003
Leneuve Patricia, Colnot Sabine, Hamard Ghislaine, Francis Fiona, Niwa-Kawakita Michiko, Giovannini Marco, Holzenberger Martin
Abstract excerpt
The binary Cre-lox conditional knockout system requires an essential part of the target gene to be flanked by loxP sites, enabling excision in vivo upon Cre expression. LoxP sites are introduced by homologous recombination, together with a selectable marker. However, this marker can disturb gene expression and should be removed. The marker is therefore often prepared with a third, flanking loxP site (tri-lox...
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