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Article

Endogenous and exogenous Na-K-Cl cotransporter expression in a low K-resistant mutant MDCK cell line.

American journal of physiology. Cell physiology - 1 Jun 2001

Payne J A, Ferrell C, Chung C Y

Abstract excerpt

A low K-resistant mutant Madin-Darby canine kidney (MDCK) cell line, LK-C1, has been shown previously to lack functional Na-K-Cl cotransporter (NKCC) activity, indicating that it may be a useful NKCC "knockout" cell line for structure-function studies. Using immunological probes, we first characterized the defect in the endogenous NKCC protein of the LK-C1 cells and then fully restored NKCC activity in these...

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Identifiers

PMID: 11350756
DOI: 10.1152/ajpcell.2001.280.6.C1607

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