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Article

C-terminal KDEL-modified cystatin C is retained in transfected CHO cells.

Biochemical and biophysical research communications - 15 Nov 1990

Johansen T E, Vogel C K, Schwartz T W

Abstract excerpt

The significance of a C-terminal tetrapeptide, Lys-Asp-Glu-Leu (KDEL), as a retention signal for the endoplasmatic reticulum was studied using cystatin C, a general thiol protease inhibitor, as the reporter protein. Clones of CHO cells were analyzed after stable transfection with eukaryotic expression vectors encoding either cystatin C, KDEL extended cystatin C, or cystatin C extended with a control sequence. It...

Topics

Amino Acid Sequence
Animals
Base Sequence
Cell Line
Chromatography, Gel
Cricetinae
Cricetulus
Cystatin C
Cystatins
DNA
Endoplasmic Reticulum
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

Transfection

Source

Identifiers

PMID: 2244918
DOI: 10.1016/0006-291x(90)91603-p

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2244918-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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