Article
A trafficking-deficient mutant of KCC3 reveals dominant-negative effects on K-Cl cotransport function.
PloS one - 1 Jan 2013
Ding Jinlong, Ponce-Coria José, Delpire Eric
Abstract excerpt
The K-Cl cotransporter (KCC) functions in maintaining chloride and volume homeostasis in a variety of cells. In the process of cloning the mouse KCC3 cDNA, we came across a cloning mutation (E289G) that rendered the cotransporter inactive in functional assays in Xenopus laevis oocytes. Through biochemical studies, we demonstrate that the mutant E289G cotransporter is glycosylation-deficient, does not move beyond...
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