Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Insertion of phosphoglycerine kinase (PGK)-neo 5' of Jlambda1 dramatically enhances VJlambda1 rearrangement.

The Journal of experimental medicine - 19 Mar 2001

Sun T, Storb U

Abstract excerpt

Gene-targeted mice were generated with a loxP-neomycin resistance gene (neo(r)) cassette inserted upstream of the Jlambda1 region and replacement of the glycine 154 codon in the Clambda1 gene with a serine codon. This insertion dramatically increases Vlambda1-Jlambda1 recombination. Jlambda1 germline transcription levels in pre-B cells and thymus cells are also greatly increased, apparently due to the strong...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Gene Rearrangement, B-Lymphocyte, Light Chain
Share to Genes, Immunoglobulin

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Immunoglobulin Joining Region

Share to Immunoglobulin lambda-Chains

Share to Immunoglobulin Variable Region

Share to Lymphocyte Count

Share to Neomycin

Share to Phosphotransferases (Alcohol Group Acceptor)

Source

Open in PubMed Open full text

Identifiers

PMID: 11257137
DOI: 10.1084/jem.193.6.699
PMCID: PMC2193413

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Insertion of phosphoglycerine kinase (PGK)-neo 5' of Jlambda1 dramatically enhances VJlambda1 rearrangement.PMID 11257137Read ↗

<i>Dlk1</i> Influences Differentiation and Function of <i>β</i> LymphocytesSemantic proximity Read ↗

B cell development arrest upon insertion of a neo gene between JH and Emu: promoter competition results in transcriptional silencing of germline JH and complete VDJ rearrangements.Semantic proximity Read ↗

Reciprocal homologous recombination in or near antibody VDJ genes.Semantic proximity Read ↗

Reverse genetic studies of homologous DNA recombination using the chicken B-lymphocyte line, DT40.Semantic proximity Read ↗

A complex major histocompatibility complex D locus variant generated by an unusual recombination mechanism in mice.Semantic proximity Read ↗

A human immunoglobulin lambda locus is similarly well expressed in mice and humans.Semantic proximity Read ↗

Assessing the pathogenic potential of the V(D)J recombinase by interlocus immunoglobulin light-chain gene rearrangement.Semantic proximity Read ↗

Promoter Proximity Defines Mutation Window for VH and VΚ Genes Rearranged to Different J Genes.Semantic proximity Read ↗

A targeted kappa immunoglobulin gene containing a deletion of the nuclear matrix association region exhibits spontaneous hyper-recombination in pre-B cells.Semantic proximity Read ↗

Mutational analyses of lymphocyte differentiation.Semantic proximity Read ↗

Overusage of mouse DH gene segment, DFL16.1, is strain-dependent and determined by cis-acting elements.Semantic proximity Read ↗

NF-kappaB elements associated with the Stat6 site in the germline gamma1 immunoglobulin promoter are not necessary for the transcriptional response to CD40 ligand.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.