Article
A targeted kappa immunoglobulin gene containing a deletion of the nuclear matrix association region exhibits spontaneous hyper-recombination in pre-B cells.
Molecular immunology - 1 Jul 1998
Hale M A, Garrard W T
Abstract excerpt
Previous studies employing ectopic integration of reporter genes have shown that the nuclear matrix association region (MAR) adjacent to the intronic enhancer of the mouse kappa immunoglobulin (Ig) gene is required for high level transcription of rearranged genes, demethylation, reduction of posi...
Topics
- Alleles
- Animals
- B-Lymphocytes
- Binding Sites
- DNA
- DNA Nucleotidyltransferases
- Enhancer Elements, Genetic
- Gene Rearrangement, B-Lymphocyte, Light Chain
- Gene Targeting
- Genes, Immunoglobulin
- Hematopoietic Stem Cells
- Immunoglobulin J-Chains
- Immunoglobulin Variable Region
- Immunoglobulin kappa-Chains
- Mice
- Nuclear Matrix
- Recombination, Genetic
- Reverse Transcriptase Polymerase Chain Reaction