Overusage of mouse DH gene segment, DFL16.1, is strain-dependent and determined by cis-acting elements.

Abstract excerpt

The DJH structure is of particular importance for diversity in the immunoglobulin heavy chain because it encodes most of CDR3. Here, we investigate mechanisms responsible for generating the DJH structure. We found DFL16.1 was used at a high frequency in normal and transformed pre-B cells (fetal liver > 50%, A-MuLV lines approximately equal to 25%). One DFL16.1JH1 structure was found repeatedly and was also...

Topics

• Alleles
• Animals
• B-Lymphocytes
• Base Sequence
• Cells, Cultured
• Chromosome Mapping
• Female
• Immunoglobulin Heavy Chains
• Liver
• Mice
• Mice, Inbred BALB C
• Mice, Inbred C57BL