Article
Genotyping of feline MHC (FLA) class II DRB by PCR-RFLP method using group-specific primers.
The Journal of veterinary medical science - 1 Dec 2000
Kuwahara Y, Kitoh K, Kobayashi R, Iwata J, Ohne R, Hosokawa-Kanai T, Matsumoto Y, Kitagawa H, Sasaki Y
Abstract excerpt
For genotyping of feline major histocompatibility complex (FLA) class II DRB, the polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) method using group-specific primers was tried. Sixty-six DRB genes were classified into 8 groups according to differences in the first 5' amino acid sequences. The group-specific primers were designed as forward ones, which were specific for 5' base...
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