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Article

DLA-DRB1 histocompatibility genotyping using RT-nested PCR and cycle sequencing.

Veterinary immunology and immunopathology - 2 Aug 1999

Happ G M, Aquilla E, Martick M, Yuncker C, Wojciechowski J, Fox L

Abstract excerpt

Class-II histocompatibility genes are associated with predisposition to autoimmune diseases in many mammal species. We have developed a technique using reverse transcriptase and nested-PCR for amplification from blood samples of expressed sequences encoded by canine DLA-DRB1 loci. In the first polymerase chain reaction (PCR), we utilize primers DR-SP and DR-STOP as developed by Sarmiento et al. (1990). In the...

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PMID: 10507296
DOI: 10.1016/s0165-2427(99)00045-8

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