Article
A functional genomics strategy that uses metabolome data to reveal the phenotype of silent mutations.
Nature biotechnology - 1 Jan 2001
Raamsdonk L M, Teusink B, Broadhurst D, Zhang N, Hayes A, Walsh M C, Berden J A, Brindle K M, Kell D B, Rowland J J, Westerhoff H V, van Dam K, Oliver S G
Abstract excerpt
A large proportion of the 6,000 genes present in the genome of Saccharomyces cerevisiae, and of those sequenced in other organisms, encode proteins of unknown function. Many of these genes are "silent, " that is, they show no overt phenotype, in terms of growth rate or other fluxes, when they are deleted from the genome. We demonstrate how the intracellular concentrations of metabolites can reveal phenotypes for...
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