Article
Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis.
Science (New York, N.Y.) - 6 Aug 1999
Winzeler E A, Shoemaker D D, Astromoff A, Liang H, Anderson K, Andre B, Bangham R, Benito R, Boeke J D, Bussey H, Chu A M, Connelly C, Davis K, Dietrich F, Dow S W, El Bakkoury M, Foury F, Friend S H, Gentalen E, Giaever G, Hegemann J H, Jones T, Laub M, Liao H, Liebundguth N, Lockhart D J, Lucau-Danila A, Lussier M, M'Rabet N, Menard P, Mittmann M, Pai C, Rebischung C, Revuelta J L, Riles L, Roberts C J, Ross-MacDonald P, Scherens B, Snyder M, Sookhai-Mahadeo S, Storms R K, Véronneau S, Voet M, Volckaert G, Ward T R, Wysocki R, Yen G S, Yu K, Zimmermann K, Philippsen P, Johnston M, Davis R W
Abstract excerpt
The functions of many open reading frames (ORFs) identified in genome-sequencing projects are unknown. New, whole-genome approaches are required to systematically determine their function. A total of 6925 Saccharomyces cerevisiae strains were constructed, by a high-throughput strategy, each with a precise deletion of one of 2026 ORFs (more than one-third of the ORFs in the genome). Of the deleted ORFs, 17 percent...
Topics
- Culture Media
- Gene Deletion
- Gene Expression Regulation, Fungal
- Gene Targeting
- Genes, Essential
- Genes, Fungal
- Genome, Fungal