Article
Concerted kinetic folding of a multidomain ribozyme with a disrupted loop-receptor interaction.
Journal of molecular biology - 5 Jan 2001
Treiber D K, Williamson J R
Abstract excerpt
The free energy landscape for the folding of large, multidomain RNAs is rugged, and kinetically trapped, misfolded intermediates are a hallmark of RNA folding reactions. Here, we examine the role of a native loop-receptor interaction in determining the ruggedness of the energy landscape for folding of the Tetrahymena ribozyme. We demonstrate a progressive smoothing of the energy landscape for ribozyme folding as...
Topics
- Animals
- Base Pairing
- Base Sequence
- Binding Sites
- Cations, Divalent
- Kinetics
- Magnesium
- Models, Chemical
- Mutation
- Nucleic Acid Conformation
- Nucleic Acid Hybridization
- Oligoribonucleotides