Article
Kinetic intermediates trapped by native interactions in RNA folding.
Science (New York, N.Y.) - 20 Mar 1998
Treiber D K, Rook M S, Zarrinkar P P, Williamson J R
Abstract excerpt
In the magnesium ion-dependent folding of the Tetrahymena ribozyme, a kinetic intermediate accumulates in which the P4-P6 domain is formed, but the P3-P7 domain is not. The kinetic barriers to P3-P7 formation were investigated with the use of in vitro selection to identify mutant RNA molecules in...
Topics
- Animals
- Binding Sites
- Kinetics
- Magnesium
- Models, Molecular
- Mutation
- Nucleic Acid Conformation
- RNA, Catalytic
- Tetrahymena