Article
Kinetic intermediates trapped by native interactions in RNA folding.
Science (New York, N.Y.) - 20 Mar 1998
Treiber D K, Rook M S, Zarrinkar P P, Williamson J R
Abstract excerpt
In the magnesium ion-dependent folding of the Tetrahymena ribozyme, a kinetic intermediate accumulates in which the P4-P6 domain is formed, but the P3-P7 domain is not. The kinetic barriers to P3-P7 formation were investigated with the use of in vitro selection to identify mutant RNA molecules in which the folding rate of the P3-P7 domain was increased. The critical mutations disrupt native tertiary interactions...
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