Improving synthetic lethal screens by regulating the yeast centromere sequence.

Abstract excerpt

The synthetic lethal screen is a useful method in identifying novel genes functioning in an alternative pathway to the gene of interest. The current synthetic lethal screen protocol in yeast is based on a colony-sectoring assay that allows direct visualization of mutant colonies among a large population by their inability to afford plasmid loss. This method demands an appropriate level of stability of the plasmid...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Aminohydrolases
• Share to Carboxy-Lyases
• Share to Centromere