Article
Genetic methods for characterizing the cis-acting components of yeast DNA replication origins.
Methods (San Diego, Calif.) - 1 Jul 1999
Huberman J A
Abstract excerpt
Small circular plasmids containing replication origins and, in some cases, centromeres, can replicate autonomously in the nuclei of all tested yeast species. Because this autonomous replication is dependent on the replication origin within the plasmid, measurements of the efficiency of autonomous replication (by the methods summarized here) permit evaluation of the effects of mutations on origin function....
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