Article
Functional and mutational analysis of conjugative transfer region 2 (Tra2) from the IncHI1 plasmid R27.
Journal of bacteriology - 1 Jan 2003
Lawley Trevor D, Gilmour Matthew W, Gunton James E, Tracz Dobryan M, Taylor Diane E
Abstract excerpt
The transfer 2 region (Tra2) of the conjugative plasmid drR27 (derepressed R27) was analyzed by PSI-BLAST, insertional mutagenesis, genetic complementation, and an H-pilus assay. Tra2 contains 11 mating-pair formation (Mpf) genes that are essential for conjugative transfer, 9 of which are essential for H-pilus production (trhA, -L, -E, -K, -B, -V, -C, -P, and -W). TrhK has similarity to secretin proteins,...
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