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Article

A rapid screen for functional mutants of TraM, an autoregulatory protein required for F conjugation.

Molecular genetics and genomics : MGG - 1 May 2003

Lu J, Fekete R A, Frost L S

Abstract excerpt

TraM is an autoregulatory protein required for conjugative transfer of the F plasmid. A rapid screening procedure was developed to select for traM mutants constructed by random PCR mutagenesis. The mutated traM gene was cloned into pT7-5, without the traM promoters (collectively called P( traM)), such that these mutants were expressed from the downstream traJ promoter, resulting in constitutive, low-level,...

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PMID: 12756534
DOI: 10.1007/s00438-003-0826-2

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Source receipt: pubmed, retrieved 7 Sept 2026.

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A rapid screen for functional mutants of TraM, an autoregulatory protein required for F conjugation.PMID 12756534Read ↗

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