Article
Enzymatic mutation detection method evaluated for detection of p53 mutations in cDNA from breast cancers.
Clinical chemistry - 1 May 2001
Norberg T, Klaar S, Lindqvist L, Lindahl T, Ahlgren J, Bergh J
Abstract excerpt
BACKGROUND: Rapid, reproducible, and easily run methods with high sensitivity and specificity are required for mutation screening of clinical samples. We evaluated the Enzymatic Mutation Detection (EMD(TM)) method by analysis of archival cDNA from 203 breast cancer patients and comparison with results of cDNA-based sequencing of the tumor suppressor gene p53. METHODS: The EMD technology uses the T4 endonuclease...
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