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Article

An endonuclease/ligase based mutation scanning method especially suited for analysis of neoplastic tissue.

Oncogene - 14 Mar 2002

Huang Jianmin, Kirk Brian, Favis Reyna, Soussi Thierry, Paty Philip, Cao Weiguo, Barany Francis

Abstract excerpt

Knowledge of inherited and sporadic mutations in known and candidate cancer genes may influence clinical decisions. We have developed a mutation scanning method that combines thermostable EndonucleaseV (Endo V) and DNA ligase. Variant and wild-type PCR amplicons are generated using fluorescently labeled primers, and heteroduplexed. Thermotoga maritima (Tma) EndoV recognizes and primarily cleaves heteroduplex DNA...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 11896624
DOI: 10.1038/sj.onc.1205109

Genes

APC
BRCA1
BRCA2
VHL

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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