Article
Detection of hotspot mutations and polymorphisms using an enhanced PCR-RFLP approach.
Human mutation - 1 May 2003
Liu W H, Kaur M, Makrigiorgos G M
Abstract excerpt
Ethidium gel-based PCR-RFLP is widely used, and is perhaps the simplest method for detection of known mutations in cancer-related genes and for genotyping a wide range of other human diseases. However, its application is limited by the fact that it can only detect mutant alleles that are present in more than 5-10% of wild-type alleles. Here we present a method that allows a 1-2 order enhancement in the...
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