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Screening for single-nucleotide polymorphisms using branch migration inhibition in PCR-amplified DNA.

Clinical chemistry - 1 Sept 2000

Lishanski A

Abstract excerpt

BACKGROUND: New methods are required for the exploration of the human genome by discovering sequence variations. This study evaluated the performance of a new method for screening a large number of samples for several DNA polymorphisms. METHODS: We used a homogeneous method based on inhibition of spontaneous branch migration by any sequence difference between two molecules of PCR-amplified DNA. A set of four PCR...

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PMID: 10973891

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