Article
PCR induction of a TaqI restriction site at any CpG dinucleotide using two mismatched primers (CpG-PCR).
Genome research - 1 Jun 1996
O'Dell S D, Humphries S E, Day I N
Abstract excerpt
A final common pathway has been devised for analysis of de novo mutation at any CpG site. Artificial restriction sites can be introduced in known DNA sequences by using either or both sense and antisense mismatched PCR primers. Sitting of the primers directly adjacent 5' and 3' to the CpG site yields a 52-bp PCR product, resulting from the sum of the two 25-mer oligonucleotides plus the two intervening bases (C...
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