Methodology for using a universal primer to label amplified DNA segments for molecular analysis.

Abstract excerpt

Detection of human DNA polymorphisms using high throughput methods often relies on generating a labeled DNA fraament which is generated by PCR using sequence-specific primers with an end labeled tag to detect a variant. The disadvantage of the synthesis of an end-labeled, sequence-specific primer to assay each DNA variant lies in the costs and time consume. In this report, we have demonstrated a methodology that...

Topics

• DNA Primers
• DNA Probes
• Fluorescent Dyes
• Gene Expression Profiling
• Microsatellite Repeats
• Molecular Diagnostic Techniques
• Nucleic Acid Amplification Techniques
• Polymerase Chain Reaction
• Polymorphism, Genetic
• Polymorphism, Single Nucleotide