Article
Overlapping PCR for bidirectional PCR amplification of specific alleles: a rapid one-tube method for simultaneously differentiating homozygotes and heterozygotes.
Genome research - 1 Apr 1997
Liu Q, Thorland E C, Heit J A, Sommer S S
Abstract excerpt
Rapid detection of single-base changes is fundamental to molecular medicine. PASA (PCR Amplification of Specific Alleles) is a rapid method of genotyping single-base changes, but one reaction is required for each allele. Bidirectional PASA (Bi-PASA) was developed to distinguish between homozygote...
Topics
- Alleles
- Catechol O-Methyltransferase
- DNA Primers
- Factor V
- Heterozygote
- Homozygote
- Humans
- Kinetics
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- Sensitivity and Specificity
- Templates, Genetic