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Article

Overlapping PCR for bidirectional PCR amplification of specific alleles: a rapid one-tube method for simultaneously differentiating homozygotes and heterozygotes.

Genome research - 1 Apr 1997

Liu Q, Thorland E C, Heit J A, Sommer S S

Abstract excerpt

Rapid detection of single-base changes is fundamental to molecular medicine. PASA (PCR Amplification of Specific Alleles) is a rapid method of genotyping single-base changes, but one reaction is required for each allele. Bidirectional PASA (Bi-PASA) was developed to distinguish between homozygotes and heterozygotes in one PCR reaction by utilizing novel primer design with appropriate cycling conditions. In...

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Identifiers

PMID: 9110178
DOI: 10.1101/gr.7.4.389
PMCID: PMC139149

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Source receipt: pubmed, retrieved 7 Sept 2026.

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