Article
Mutational and nonmutational activation of p21ras in rat colonic azoxymethane-induced tumors: effects on mitogen-activated protein kinase, cyclooxygenase-2, and cyclin D1.
Cancer research - 15 Aug 2000
Bissonnette M, Khare S, von Lintig F C, Wali R K, Nguyen L, Zhang Y, Hart J, Skarosi S, Varki N, Boss G R, Brasitus T A
Abstract excerpt
Azoxymethane (AOM)-induced colonic carcinogenesis involves a number of mutations, including those in the K-ras gene and CTNNB1, that codes for beta-catenin. Prior in vitro studies have also demonstrated that wild type p21(K-ras) can be activated by epigenetic events. We identified 15 K-ras mutations in 14 of 84 AOM-induced colonic tumors by three independent methods. By single strand conformational polymorphism,...
Topics
- Animals
- Azoxymethane
- Carcinogens
- Colonic Neoplasms
- Cyclin D1
- Cyclooxygenase 2
- Cytoskeletal Proteins
- Enzyme Activation
- Genes, ras
- Isoenzymes