Article
The RAS oncogene induces genomic instability in thyroid PCCL3 cells via the MAPK pathway.
Oncogene - 10 Aug 2000
Saavedra H I, Knauf J A, Shirokawa J M, Wang J, Ouyang B, Elisei R, Stambrook P J, Fagin J A
Abstract excerpt
Activating mutations of RAS are thought to be early events in the evolution of thyroid follicular neoplasms. We used a doxycycline-inducible expression system to explore the acute effects of H-RAS12 on genomic stability in thyroid PCCL3 cells. At 2-3 days (first or second cell cycle) there was a significant increase in the frequency of micronucleation. Treatment of cells with YVAD-CHO inhibited RAS-induced...
Topics
- Animals
- Apoptosis
- Cell Nucleus
- Chromosomal Proteins, Non-Histone
- Doxycycline
- Enzyme Inhibitors
- Flavonoids
- Gamma Rays
- Gene Expression Regulation, Neoplastic
- MAP Kinase Kinase 1
- MAP Kinase Signaling System
- Mitogen-Activated Protein Kinase Kinases
- Mutation