Article
Conditional immortalization of human thyroid epithelial cells: a tool for analysis of oncogene action.
Molecular and cellular biology - 1 Oct 1990
Wynford-Thomas D, Bond J A, Wyllie F S, Burns J S, Williams E D, Jones T, Sheer D, Lemoine N R
Abstract excerpt
To overcome the difficulty of assessing oncogene action in human epithelial cell types, such as thyroid, which have limited proliferative potential in culture, we have explored the use of temperature-sensitive (ts) mutants of simian virus 40 (SV40) early region to create conditionally immortalized epithelial cell lines. Normal primary cultures of human thyroid follicular cells were transfected with a plasmid...
Topics
- Animals
- Antigens, Viral, Tumor
- Blotting, Northern
- Cell Adhesion
- Cell Differentiation
- Cell Division
- Cell Transformation, Neoplastic
- Clone Cells
- Epithelial Cells
- Gene Expression
- Humans