Article
The internal phosphodiesterase RegA is essential for the suppression of lateral pseudopods during Dictyostelium chemotaxis.
Molecular biology of the cell - 1 Aug 2000
Wessels D J, Zhang H, Reynolds J, Daniels K, Heid P, Lu S, Kuspa A, Shaulsky G, Loomis W F, Soll D R
Abstract excerpt
Dictyostelium strains in which the gene encoding the cytoplasmic cAMP phosphodiesterase RegA is inactivated form small aggregates. This defect was corrected by introducing copies of the wild-type regA gene, indicating that the defect was solely the consequence of the loss of the phosphodiesterase. Using a computer-assisted motion analysis system, regA(-) mutant cells were found to show little sense of direction...
Topics
- 3',5'-Cyclic-AMP Phosphodiesterases
- Animals
- Cell Aggregation
- Chemotaxis
- Computer Simulation
- Cyclic AMP
- Cyclic AMP-Dependent Protein Kinases
- Dictyostelium
- Genes, Protozoan
- Mutation
- Myosins
- Protozoan Proteins
- Pseudopodia