Article
Priming of PC12 cells for semiquantitative microinjection studies involving Ras.
FEBS letters - 2 Jun 2000
Schmidt G, Wittinghofer A
Abstract excerpt
Nerve growth factor and activated Ras can induce differentiation of rat pheochromocytoma cells (PC12 cells) [Greene and Tischler (1976) Proc. Natl. Acad. Sci. USA 73, 2424-2428] from a chromaffin cell-like morphology into one that resembles sympathetic neurones. We developed a special treatment of PC12 cells which apparently synchronizes these cells such that they are more useful for semi-quantitative...
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