Article
A PC12 variant lacking regulated secretory organelles: aberrant protein targeting and evidence for a factor inhibiting neuroendocrine gene expression.
Journal of neurochemistry - 1 Jul 1999
Pance A, Morgan K, Guest P C, Bowers K, Dean G E, Cutler D F, Jackson A P
Abstract excerpt
A variant of the PC12 pheochromocytoma cell line (termed A35C) has been isolated that lacks regulated secretory organelles and several constituent proteins. Northern and Southern blot analyses suggested a block at the transcriptional level. The proprotein-converting enzyme carboxypeptidase H was synthesised in the A35C cell line but was secreted by the constitutive pathway. Transient transfection of A35C cells...
Topics
- Animals
- Calcium-Binding Proteins
- Carboxypeptidase H
- Carboxypeptidases
- Clathrin
- Dopamine beta-Hydroxylase
- Gene Expression
- Gene Targeting
- Hybrid Cells
- Membrane Glycoproteins
- Microscopy, Electron
- Mutation
- Nerve Tissue Proteins