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Article

One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products.

Proceedings of the National Academy of Sciences of the United States of America - 6 Jun 2000

Datsenko K A, Wanner B L

Abstract excerpt

We have developed a simple and highly efficient method to disrupt chromosomal genes in Escherichia coli in which PCR primers provide the homology to the targeted gene(s). In this procedure, recombination requires the phage lambda Red recombinase, which is synthesized under the control of an inducible promoter on an easily curable, low copy number plasmid. To demonstrate the utility of this approach, we generated...

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Identifiers

PMID: 10829079
DOI: 10.1073/pnas.120163297
PMCID: PMC18686

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Source receipt: pubmed, retrieved 7 Sept 2026.

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