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Article

The construction of systematic in-frame, single-gene knockout mutant collection in Escherichia coli K-12.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2008

Baba Tomoya, Mori Hirotada

Abstract excerpt

Here we describe the systematic construction of well-defined, in-frame, single-gene deletions of all nonessential genes in Escherichia coli K-12. The principal strategy is based on the method for one-step inactivation of chromosomal genes in E. coli K-12 established by Datsenko and Wanner (1), namely, the replacement of a target gene with a selectable antibiotic-resistant marker generated by polymerase chain...

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PMID: 18392967
DOI: 10.1007/978-1-59745-321-9_11

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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