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Article

A rapid and simple method for inactivating chromosomal genes in Yersinia.

FEMS immunology and medical microbiology - 22 Sept 2003

Derbise Anne, Lesic Biliana, Dacheux Denis, Ghigo Jean Marc, Carniel Elisabeth

Abstract excerpt

A polymerase chain reaction (PCR)-based procedure without any cloning step was developed for a rapid mutagenesis/deletion of chromosomal target genes in Yersinia. For this purpose, a PCR fragment carrying an antibiotic resistance gene flanked by regions homologous to the target locus is electroporated into a recipient strain expressing the highly proficient homologous recombination system encoded by plasmid...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 13129645
DOI: 10.1016/S0928-8244(03)00181-0

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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