Rapid allelic exchange in enterohemorrhagic Escherichia coli (EHEC) and other E. coli using lambda red recombination.

doi:10.1002/9780471729259.mc05a02s00

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Article

Rapid allelic exchange in enterohemorrhagic Escherichia coli (EHEC) and other E. coli using lambda red recombination.

Current protocols in microbiology - 1 Jan 2006

Savage Pamela J, Leong John M, Murphy Kenan C

Abstract excerpt

This unit describes an allelic exchange system for enterohemorrhagic E. coli (EHEC), and similar pathogenic species of bacteria. The phage lambda Red recombination system is expressed from a plasmid, inducing a hyper-recombinogenic state where electroporated PCR-generated substrates recombine with the bacterial chromosome at high efficiency. The technique can be used to substitute a drug marker for the gene of...

Topics

Alleles
Bacteriophage lambda
DNA, Bacterial
DNA, Recombinant
Enterohemorrhagic Escherichia coli
Mutagenesis, Site-Directed
Recombination, Genetic

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PMID: 18770591
DOI: 10.1002/9780471729259.mc05a02s00

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