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Article

Application of lambda Red recombination system to Vibrio cholerae genetics: simple methods for inactivation and modification of chromosomal genes.

Gene - 1 Jun 2009

Yamamoto Shouji, Izumiya Hidemasa, Morita Masatomo, Arakawa Eiji, Watanabe Haruo

Abstract excerpt

The lambda Red-based recombination system is very useful for genetic manipulation of some Gram-negative bacteria. Here we report simple procedures for the inactivation and modification of genes of interest on Vibrio cholerae chromosome using this recombination technique. For this purpose, a polymerase chain reaction (PCR) fragment carrying an antibiotic resistance cassette flanked by regions homologous to the...

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Identifiers

PMID: 19268696
DOI: 10.1016/j.gene.2009.02.015

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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