Article
Lambda Red-mediated recombinogenic engineering of enterohemorrhagic and enteropathogenic E. coli.
BMC molecular biology - 13 Dec 2003
Murphy Kenan C, Campellone Kenneth G
Abstract excerpt
BACKGROUND: The lambda Red recombineering technology has been used extensively in Escherichia coli and Salmonella typhimurium for easy PCR-mediated generation of deletion mutants, but less so in pathogenic species of E. coli such as EHEC and EPEC. Our early experiments with the use of lambda Red in EHEC and EPEC have led to sporadic results, leading to the present study to identify factors that might improve the...
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