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Article

High yield purification and physico-chemical properties of full-length recombinant allelic variants of sheep prion protein linked to scrapie susceptibility.

European journal of biochemistry - 1 May 2000

Rezaei H, Marc D, Choiset Y, Takahashi M, Hui Bon Hoa G, Haertlé T, Grosclaude J, Debey P

Abstract excerpt

Sheep susceptibility to scrapie is governed by polymorphisms at two major sites, codons 136 and 171, of the prp gene. To get more insight into the prion protein (PrP) sequence-linked basis of differential scrapie susceptibility, a high yield one-step method for the purification (over 99% final purity) of the full-length recombinant sheep PrP was developed, based on the affinity of the conserved octapeptide...

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Identifiers

PMID: 10806380
DOI: 10.1046/j.1432-1033.2000.01347.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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