Article
Characterization of novel rad6/ubc2 ubiquitin-conjugating enzyme mutants in yeast.
Current genetics - 1 Apr 2000
Freiberg G, Mesecar A D, Huang H, Hong J Y, Liebman S W
Abstract excerpt
Null mutations in the RAD6/UBC2 gene encoding an E2 ubiquitin-conjugating enzyme cause deficiencies in DNA repair, N-end-rule protein degradation, sporulation and telomeric silencing, and alter the preferred integration positions for Ty1 retrotransposons. Here we selected for mutants of RAD6 that cause a release of telomeric silencing. Some alleles retained nearly wild-type ability for sporulation, DNA repair and...
Topics
- Alleles
- Blotting, Western
- Crystallography, X-Ray
- DNA Repair
- Gene Silencing
- Ligases
- Models, Molecular
- Mutation
- Retroelements
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Spores, Fungal
- Telomere
- Ubiquitin-Conjugating Enzymes
- Ubiquitins
- Ultraviolet Rays