A site-directed approach for constructing temperature-sensitive ubiquitin-conjugating enzymes reveals a cell cycle function and growth function for RAD6.

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Article

A site-directed approach for constructing temperature-sensitive ubiquitin-conjugating enzymes reveals a cell cycle function and growth function for RAD6.

The Journal of biological chemistry - 15 Dec 1991

Ellison K S, Gwozd T, Prendergast J A, Paterson M C, Ellison M J

Abstract excerpt

We have determined the gene sequence of a temperature-sensitive allele of the cell cycle-related ubiquitin-conjugating enzyme CDC34 (UBC 3) from Saccharomyces cerevisiae. The basis of temperature sensitivity is a missense mutation resulting in a proline to serine substitution at a residue that is...

Topics

Alleles
Amino Acid Sequence
Anaphase-Promoting Complex-Cyclosome
Base Sequence
Cell Cycle
Cell Division
Fungal Proteins
Genes, Fungal
Humans
Ligases
Molecular Sequence Data
Mutagenesis, Site-Directed

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Oligodeoxyribonucleotides

Restriction Mapping

Saccharomyces cerevisiae

Saccharomyces cerevisiae Proteins

Sequence Homology, Nucleic Acid

Temperature

Source

Open in PubMed

Identifiers

PMID: 1748683

Genes

CDC34
UBC

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-1748683-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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