Article
Respective roles of cyclobutane pyrimidine dimers, (6-4)photoproducts, and minor photoproducts in ultraviolet mutagenesis of repair-deficient xeroderma pigmentosum A cells.
Cancer research - 15 Mar 2000
Otoshi E, Yagi T, Mori T, Matsunaga T, Nikaido O, Kim S T, Hitomi K, Ikenaga M, Todo T
Abstract excerpt
The role of UV light-induced photoproducts in initiating base substitution mutation in human cells was examined by determining the frequency and spectrum of mutation in a supF tRNA gene in a shuttle vector plasmid transfected into DNA repair deficient cells (xeroderma pigmentosum complementation group A). To compare the role of two major UV-induced photoproducts, cis-syn cyclobutane-type pyrimidine dimers (CPDs)...
Topics
- Base Sequence
- Cell Line, Transformed
- DNA
- DNA Mutational Analysis
- DNA Repair
- Enzyme-Linked Immunosorbent Assay
- Genes, Suppressor
- Humans
- Molecular Sequence Data
- Mutagenesis
- Mutation
- Plasmids
- Point Mutation