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Article

A food-grade cloning system for industrial strains of Lactococcus lactis.

Applied and environmental microbiology - 1 Apr 2000

Sørensen K I, Larsen R, Kibenich A, Junge M P, Johansen E

Abstract excerpt

We have previously reported the construction of a food-grade cloning vector for Lactococcus using the ochre suppressor, supB, as the selective marker. This vector, pFG1, causes only a slight growth inhibition in the laboratory strain MG1363 but is unstable in the industrial strains tested. As supB suppresses both amber and ochre stop codons, which are present in 82% of all known lactococcal genes, this...

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Identifiers

PMID: 10742196
DOI: 10.1128/AEM.66.4.1253-1258.2000
PMCID: PMC91977

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Source receipt: pubmed, retrieved 7 Sept 2026.

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