Construction of a food-grade host/vector system for Lactococcus lactis based on the lactose operon.

doi:10.1111/j.1574-6968.1995.tb07457.x

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Article

Construction of a food-grade host/vector system for Lactococcus lactis based on the lactose operon.

FEMS microbiology letters - 15 Mar 1995

MacCormick C A, Griffin H G, Gasson M J

Abstract excerpt

A plasmid-based food-grade vector system was developed for Lactococcus lactis by exploiting the genes for lactose metabolism. L. lactis MG5267 is a plasmid-free strain containing the entire lactose operon as a chromosomal insertion. The lacF gene was deleted from this strain by a double cross-ove...

Topics

Base Sequence
DNA Primers
Food Microbiology
Gene Deletion
Genetic Complementation Test
Genetic Vectors
Lac Operon
Lactococcus lactis
Molecular Sequence Data
Phenotype
Plasmids

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