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Article

Construction of a food-grade host/vector system for Lactococcus lactis based on the lactose operon.

FEMS microbiology letters - 15 Mar 1995

MacCormick C A, Griffin H G, Gasson M J

Abstract excerpt

A plasmid-based food-grade vector system was developed for Lactococcus lactis by exploiting the genes for lactose metabolism. L. lactis MG5267 is a plasmid-free strain containing the entire lactose operon as a chromosomal insertion. The lacF gene was deleted from this strain by a double cross-over homologous recombination event. The lacF-deficient strain produced a Lac- phenotype on indicator agar. A cloned copy...

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Source

Identifiers

PMID: 7737470
DOI: 10.1111/j.1574-6968.1995.tb07457.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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