Isolation of Lactococcus lactis nonsense suppressors and construction of a food-grade cloning vector.

doi:10.1111/j.1365-2958.1995.tb02354.x

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Back to search

Article

Isolation of Lactococcus lactis nonsense suppressors and construction of a food-grade cloning vector.

Molecular microbiology - 1 Mar 1995

Dickely F, Nilsson D, Hansen E B, Johansen E

Abstract excerpt

Nonsense suppressor strains of Lactococcus lactis were isolated using plasmids containing nonsense mutations or as revertants of a nonsense auxotrophic mutant. The nonsense suppressor gene was cloned from two suppressor strains and the DNA sequence determined. One suppressor is an ochre suppressor with an altered tRNA(gln) and the other an amber suppressor with an altered tRNA(ser). The nonsense suppressors...

Topics

Alleles
Aminopeptidases
Bacteriophages
Base Sequence
Cloning, Molecular
Codon, Nonsense
DNA Primers
Gene Dosage
Gene Expression Regulation, Bacterial
Genes, Suppressor
Genetic Vectors

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Lactococcus lactis

Molecular Sequence Data

Mutagenesis

Promoter Regions, Genetic

RNA, Transfer

Sequence Analysis

Source

Open in PubMed

Identifiers

PMID: 7596286
DOI: 10.1111/j.1365-2958.1995.tb02354.x

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7596286-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Isolation of Lactococcus lactis nonsense suppressors and construction of a food-grade cloning vector.PMID 7596286Read ↗

A food-grade cloning system for industrial strains of Lactococcus lactis.Semantic proximity Read ↗

Plasmid-encoded determinants for bacteriocin production and immunity in a Lactococcus lactis strain and purification of the inhibitory peptide.Semantic proximity Read ↗

A gene replacement strategy for engineering nisin.Semantic proximity Read ↗

Regulation of the nisin operons in Lactococcus lactis N8.Semantic proximity Read ↗

Genetics of peptide degradation in Lactococcus: gene cloning, construction and analysis of peptidase negative mutants.Semantic proximity Read ↗

pKSS--a second-generation general purpose cloning vector for efficient positive selection of recombinant clones.Semantic proximity Read ↗

Identification of a nucleotide sequence conserved in Lactococcus lactis bacteriophages.Semantic proximity Read ↗

Cloning, sequencing, and expression in Escherichia coli of lcnB, a third bacteriocin determinant from the lactococcal bacteriocin plasmid p9B4-6.Semantic proximity Read ↗

Efficient transformation of Lactococcus lactis IL1403 and generation of knock-out mutants by homologous recombination.Semantic proximity Read ↗

Cloning and analysis of the leuB gene of Leptospira interrogans serovar pomona.Semantic proximity Read ↗

Construction and application of a food-grade expression system for Lactococcus lactis.Semantic proximity Read ↗

Gene selective suppression of nonsense termination using antisense agents.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.