Article
Multiple-peptidase mutants of Lactococcus lactis are severely impaired in their ability to grow in milk.
Journal of bacteriology - 1 May 1996
Mierau I, Kunji E R, Leenhouts K J, Hellendoorn M A, Haandrikman A J, Poolman B, Konings W N, Venema G, Kok J
Abstract excerpt
To examine the contribution of peptidases to the growth of lactococcus lactis in milk, 16 single- and multiple-deletion mutants were constructed. In successive rounds of chromosomal gene replacement mutagenesis, up to all five of the following peptidase genes were inactivated (fivefold mutant): p...
Topics
- Amino Acid Sequence
- Amino Acids
- Animals
- Bacterial Proteins
- Base Sequence
- Biological Transport
- Blotting, Southern
- Blotting, Western
- Carrier Proteins
- Caseins
- Cloning, Molecular
- Endopeptidases
- Lactococcus lactis
- Lipoproteins
- Milk