Article
V(D)J recombination catalyzed by mutant RAG proteins lacking consensus DNA-PK phosphorylation sites.
Molecular immunology - 1 Dec 1999
Lin J M, Landree M A, Roth D B
Abstract excerpt
The process of antigen receptor gene rearrangement, V(D)J recombination, involves DNA cleavage by the RAG-1 and RAG-2 proteins. Cleavage generates covalently sealed (hairpin) DNA ends, termed coding ends, which must be opened by an endonuclease prior to joining. Resolution of these hairpin ends requires the activity of the DNA-dependent protein kinase (DNA-PK), a protein kinase whose specific role is yet...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Binding Sites
- Conserved Sequence
- DNA
- DNA Primers
- DNA-Activated Protein Kinase
- DNA-Binding Proteins
- Gene Rearrangement
- Gene Rearrangement, B-Lymphocyte
- Gene Rearrangement, T-Lymphocyte
- Genes, RAG-1
- Homeodomain Proteins
- Humans
- In Vitro Techniques
- Molecular Sequence Data
- Mutagenesis, Site-Directed