Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Identification and characterization of a gain-of-function RAG-1 mutant.

Molecular and cellular biology - 1 Jun 2006

Kriatchko Aleksei N, Anderson Dirk K, Swanson Patrick C

Abstract excerpt

RAG-1 and RAG-2 initiate V(D)J recombination by cleaving DNA at recombination signal sequences through sequential nicking and transesterification reactions to yield blunt signal ends and coding ends terminating in a DNA hairpin structure. Ubiquitous DNA repair factors then mediate the rejoining of broken DNA. V(D)J recombination adheres to the 12/23 rule, which limits rearrangement to signal sequences bearing...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Mutagenesis, Site-Directed
Share to Genes, RAG-1
Share to Homeodomain Proteins

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to In Vitro Techniques

Share to Kinetics

Share to Nucleic Acid Conformation

Share to Recombinant Proteins

Share to Recombination, Genetic

Source

Open in PubMed Open full text

Identifiers

PMID: 16738334
DOI: 10.1128/MCB.02487-05
PMCID: PMC1489120

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Identification and characterization of a gain-of-function RAG-1 mutant.PMID 16738334Read ↗

RAG-heptamer interaction in the synaptic complex is a crucial biochemical checkpoint for the 12/23 recombination rule.Semantic proximity Read ↗

Full-length RAG1 promotes contact with coding and intersignal sequences in RAG protein complexes bound to recombination signals paired in cis.Semantic proximity Read ↗

Understanding how the V(D)J recombinase catalyzes transesterification: distinctions between DNA cleavage and transposition.Semantic proximity Read ↗

Leaky severe combined immunodeficiency and aberrant DNA rearrangements due to a hypomorphic RAG1 mutation.Semantic proximity Read ↗

Initiation of V(D)J recombination in vitro obeying the 12/23 rule.Semantic proximity Read ↗

The DDE motif in RAG-1 is contributed in trans to a single active site that catalyzes the nicking and transesterification steps of V(D)J recombination.Semantic proximity Read ↗

Requirements for DNA hairpin formation by RAG1/2.Semantic proximity Read ↗

Role of recombination activating genes in the generation of antigen receptor diversity and beyond.Semantic proximity Read ↗

Assembly of a 12/23 paired signal complex: a critical control point in V(D)J recombination.Semantic proximity Read ↗

Molecular Mechanism of V(D)J Recombination from Synaptic RAG1-RAG2 Complex Structures.Semantic proximity Read ↗

Mutational analysis of all conserved basic amino acids in RAG-1 reveals catalytic, step arrest, and joining-deficient mutants in the V(D)J recombinase.Semantic proximity Read ↗

Impairment of V(D)J recombination in double-strand break repair mutants.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.